Abstract
This study determined lethal dose levels of road salt on selected macroinvertebrates from a Michigan, USA wetland under different testing conditions and related those levels to in situ chloride concentrations of standing water habitats along multi-lane highways. We conducted simultaneous acute (24 and 96 h) and chronic (15 d) toxicity experiments using laboratory containers, containers adjacent to a wetland, and in situ modular field PVC microcosms. Data showed that Callibaetis fluctuans, Physella integra, Hyallela azteca, and Chaoborus americamus had relatively high tolerance to elevated road-salt levels. Depending on test condition, road-salt 96 h LC50 estimates ranged from greater than 5000 mg 1−1 (2558 mg 1−1 Cl−) to >10,000 mg 1−1 (4502 mg 1−1 Cl−) road salt for C. fluctuans and P. integra, respectively. For H. azteca and C. americanus, mortality was generally low at all salt concentrations, with road-salt 96 h LC50 estimates >10,000 mg 1−1, Chloride concentrations of 43 impacted Michigan wetlands ranged from to 2700 mg 1−1 Cl−1, with 75% <334 mg 1−1, indicating that macroinvertebrate LC50 chloride estimates for the study species are well above most concentrations of the 43 wetlands.
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Literature Cited
Berner, L. and W. C. Sloan. 1954. The occurrence of a mayfly nymph in brackish water. Ecology 35:98.
Berner, L. and M. L. Pescador. 1988. The Mayflies of Florida, revised edition. University of Florida Press. Tallahassee, FL, USA.
Blasius, B. J. and R. W. Merritt 2002. Field and laboratory investigations on the effects of road-salt (NaCl) on stream macroinvertebrate communities. Environmental Pollution 120:219–231.
Boyle, T. P., S. E. Finger, R. L. Paulson, and C. F. Rabeni. 1985. Comparison of laboratory and field assessment of fluorene-Part II: Effects on the ecological structure and function of experimental pond ecosystems, p. 134–151. In T. P. Boyle (ed.) Validation and Predietability of Laboratory Methods for Assessing the Fate and Effects of Contaminants in Aquatic Ecosysems. American Society for Testing and Materials, Philadelphia, PA, USA.
Bubeck, R. C., W. H. Diment, B. L. Deck, A. L. Baldwin, and S. D. Lipton. 1971. Runoff of deicing salt: effects on Irondequoit Bay, Rochester, New York. Science 172:1128–1132.
Cairns, J., Jr. and B. R. Niederlehner. 1987. Problems associated with selecting the most sensitive species for toxicity testing. Hydrobiologia 153:87–94.
Chadwick M. A. 1997. Influences of seasonal salimity and temperature on Hexagenia limbata (Serville) (Ephemeroptera: Ephemeridae) in the Mobile River. M.S. Thesis. Auburn University, Auburn, AL, USA.
Crowther, R. A. and H. B. N. Hynes. 1977. The effect of road deicing salt on the drift of stream benthos. Environmental Pollution 14:113–126.
D’Itri, F. M. 1992. Chemical Deicers and he Environment. Lewis Publishers, Inc. Ann Arbor, MI, USA.
Demers, C. L. 1992. Effects of road deicing salt on aquatic invertebrates in four Adirondack streams, p. 245–251. In F. M. D’Itri (ed.) Chemical Deicers and the Environment. Lewis Publishers, Ann Arbor, MI, USA.
Environment Canada 2001. Priority substances list assessment report—road-salts. Environment Canada, Health Canada, Minister of Public Works and Government Services 2001, Ottawa, ON, Canada.
Field, R. and M. L. O’Shea 1992. The USEPA research program on the environmental impacts and control of highway deicing salt pollution, p. 117–133. In F. M. D’itri (ed.) Chemical Deicers and the Environment. Lewis Publishers, Inc., Ann Arbor, MI, USA.
Giberson, D. J., B. Bilyj, and N. Burgess. 2001. Species diversity and emergence patterns of Nematocerous flies (Insecta: Diptera) from three coastal salt marshes in Prince Edward Island. Canada. Estuaries 24:862–874.
Goetsch, P. and C. G. Palmer. 1997. Salinity tolerances of selected macroinvertebrates of the Sabie River, Kruger National Park, South Africa. Archives of Environmental Contamination and Toxicology 32:32–41.
Hanazato, T. and M. Yasuno. 1989. Effects of temperature in laboratory studies on growth of Chaoborus flavicans (Diptera: Chaoboridae). Archiv fur Hydrobiologie Stuttgart 114:497–504.
Hart, B. T., P. Bailey, R. Edwards, K. Hortle, K. James, and A. McMahon. 1991. A review of the salt sensitivity of the Australian freshwater biota. Hydrobiologia 210:105–144.
Hawkins, R. H. and J. H. Judd. 1972. Water pollution as affected by street salting. Water Resources Bulletin 8:1246–1252.
Hoffman, R. W., G. R. Goldman, S. Paulson, and G. R. Winters. 1981. Aquatic impacts of deicing salts in the Central Sierra Nevada Mountains, California. Water Resources Bulletin 17:280–285.
Judd, J. H. 1969. Effect of runoff from street deicing on a small lake. Ph. D. Dissertation. University of Wisconsin, Madison, WI, USA.
Judd, J. H. 1970. Lake stratification caused by runoff from street deicing. Water Research 4:521–532.
Kelts, L. J. 1979. Ecology of a tidal marsh corixid, Trichocorixa verticalis (Insecta: Hemiptera). Hydrobiologia 64:37–57.
Kimball, K. D. and S. A. Levin. 1985. Limitations of laboratory bioassays: the need for ecosystem-level testing. Bioscience 35: 165–171.
Kjensmo, J 1997. The influence of road-salts on the salinity and the meromictic stability of Lake Svinsjoen, southeastern Norway. Hydrobiologia 347:151–158.
LaRow, E. J. 1970. The effect of oxygen tension on the vertical migration of Chaoborus larvae. Limology and Oceanography 15: 357–362.
Lathrop, R. C. 1975. A limnological study of two neighboring lakes: First Sister Lake and Second Sister Lake, Washtenaw County, Michigan, M. S. Thesis. The University of Michigan, Ann Arbor, MI, USA.
Limno-Tech, Inc. 1997. First and Second Sister Lakes: diagnostic/feasibility study. Final Report to the City of Ann Arbor Department of Parks and Recreation. Ann Arbor, MI, USA.
Mayer, T., W. J. Snodgrass, and D. Morin. 1999. Spatial characterization of the occurrence of road-salts and their environmental concentrations as chlorides in Canadian surface waters and benthic sediments. Water Quality Research 34:545–574.
Motulsky, H. J. 1999. Analyzing Data with GraphPad Prism. GraphPad Software, Inc., San Diego, CA, USA.
Nelson, J. W., D. A. Armstrong, A. W. Knight, and H. W. Li. 1977. The effects of temperature and salinity on metabolic rate of juvenile Macrobrachium rosenbergii (Crustacea: Palaemonidae). Comparative Biochemistry and Physiology 56A:533–537.
Ohno, T. 1990. Levels of cyanide and NaCl in surface waters adjacent to road-salt facilities. Environmental Pollution 67:123–132.
Pennak, R. W. 1989. Freshwater Invertebrates of the United States, Protozoa to Mollusca. John Wiley & Sons, Inc., New York, NY, USA.
Peters, N. E. and J. T. Turk. 1981. Increases in sodium and chloride in the Mohawk River, New York, from the 1950s to the 1970s attributed to road-salt. Water Resources Bulletin 17:586–598.
Pontasch, K. W., B. R. Niederlehner, and J. Cairns, Jr. 1989. Comparisons of single-species, microcosm and field responses to a complex effluent. Environmental Toxicology and Chemistry 8:521–532.
Rand, G. M., P. G. Wells, and L. S. McCarty. 1995. Introduction to aquatic Toxicology, p. 3–67. In G. M. Rand (ed.) Fundamentals of Aquatic Toxicology, 2nd ed. Taylor & Francis. Washington, DC, USA.
Sutcliffe, D. W. 1961a. Studies on salt and water balance in caddis larvae (Trichoptera): I. Osmotic and ionic regulation of body fluids in Limnephilus affinis Curtis. Journal of Experimental Biology 38:501–519.
Sutcliffe, D. W. 1961b. Studies on salt and water balance in caddis larvae (Trichoptera): II. Osmotic and ionic regulation of body fluids in Linnephilus stigma Curtis and Anabolia nervosa Leach. Journal of Experimental Biology 38:521–530.
Wichard, W. 1975. Osmoregulatory adaptations of aquatic insects in the lake district ‘Neusiedlersee’. Nachrichtenblatt der Bayerischen Entomologen 24:81–87.
Wilcox, D. A. 1986. The effects of deicing salts on vegetation in Pinhook Bog, Indiana. Water Resources Bulletin 64:865–874.
Williams, D. D., N. E. William, and Y. Cao. 1997. Spatial differences in macroinvertebrate community structure in springs in southeastern Ontario in relation to their chemical and physical environments. Canadian Journal of Zoology 75:1404–1414.
Williams, D. D. and N. E. Williams. 1998a. Seasonal variation, export dynamics and consumption of freshwater invertebrates in an estuarine environment. Estuarine, Coastal and Shelf Science 46:393–410.
Williams, D. D. and N. E. Williams. 1998b. Aquatic insects in an estuarine environment: densities, distribution and salinity tolerance. Freshwater Biology 39:411–421.
Williams, D. D., N. E. Williams, and Y. Cao. 2000. Road-salt contanination of groundwater in a major metropolitan area and development of a biological index to monitor its impact. Water Research 34:127–138.
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Benbow, M.E., Merritt, R.W. Road-salt toxicity of select Michigan wetland macroinvertebrates under different testing conditions. Wetlands 24, 68–76 (2004). https://doi.org/10.1672/0277-5212(2004)024[0068:RTOSMW]2.0.CO;2
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DOI: https://doi.org/10.1672/0277-5212(2004)024[0068:RTOSMW]2.0.CO;2